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KMID : 1123920050190030743
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Korean Journal of Oriental Physiology and Pathology
2005 Volume.19 No. 3 p.743 ~ p.748
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Role of K+ Channels in the Vasodilation of Jagumhuan
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Son Chang-Woo
Lee Heon-Jae
Liang Liou Jia
Shin Heung-Mook
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Abstract
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This study was performed for the investigation of vasodilatory efficacy and its underlying mechanisms of Jagumhuan(JGH), a herbal remedy. JGH produced completely endothelium-dependent relaxation and relaxed phenylephrine(PE)-precontracted aorta in a concentration dependent manner. The magnitude of relaxation was greater in PE induced contraction than that of KCl, suggesting involvement of channel in the relaxant effect. Both glibenclamide, a channel inhibitor and indometacin, a cyclooxygenase inhibitor, completely prevented this relaxation. The relaxation effects of JGH, involve in part the release of nitric oxide from the endothelium as pretreatment with L-NAME, an NOS inhibitor, and methylene blue, a cGMP inhibitor, attenuated the responses by 62% and 58%, respectively. In addition, nitrite was produced by JGH in human aortic smooth muscle cells and human umbilical vein endothelial cells. The relaxant effect of JGH was also inhibited by 55.41% by tetraethylammonium(TEA; 5mM), a channel inhibitor. In the absence of extracellular , pre-incubation of the aortic rings with JGH significantly reduced the contraction by PE, suggesting that the relaxant action of the JGH includes inhibition of release from intracellular stores. These results indicate that in rat thoracic aorta, JGH may induce vasodilation through ATP sensitive channel activation by prostacyclin production. However, the relaxant effect of JGH may also mediated in part by NO pathways and activated channel.
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KEYWORD
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Jagumhuan, Vasodilation, ATP sensitive K+ channel, Ca2+ release
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